Description
Molecular weight (MW) and its distribution are important characteristics of heparins, a family of anticoagulants with polydisperse, heterogeneous polysaccharide chains. Current compendial methods for heparin molecular weight determinations involve size-exclusion chromatography (SEC).
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution.
The combination of BioCore SEC-300HP and SEC-500HP analytical columns is recommended for molecular weight determinations of unfractionated heparin.
System suitability for unfractionated heparin
Heparin polysaccharide chains are hard to separation. With theoretical plate number per meter > 100,000, BioCore SEC-HP series SEC columns feature great column performance that can address this separation issue.
With BioCore SEC-300HP and SEC-500HP analytical columns, a correlation coefficient great than 0.9999 can be easily obtained in the calibration curve relating molecular weight to retention time.
Great durability with over 100 injections
Heparin samples are very complex and challenge column durability. As shown in the figure bellow, with BioCore SEC-150HP analytical column, minimal variation between 30 injections of LMWHs samples can be observed.
According to feedbacks from customers, BioCore SEC-150HP can be used over 100 injections. This durability reduces your cost per injection to 1/3 to 1/4, compared to SEC columns from other vendors.
Properties:
| Separation mechanism | Size-exclusion chromatography (SEC) |
Packaging type | USP L59 |
|---|---|---|---|
| I.D. | 7.8 mm | Length | 300 mm |
| Matrix | Monodisperse silica particle | Functional group | Diol |
| Pore size | 300 Ã… (for SEC-300HP) 500 Ã… (for SEC-500HP) |
Particle size | 5 μm |
| pH range | 2 – 8 | Maximum pressure | 6,000 psi |
| Calibration range (globular proteins) |
10,000 – 1,000,000 Da (for SEC-300) 20,000 – 2,000,000 Da (for SEC-500) |
Calibration range (glycosaminoglycans) |
5,000 – 150,000 Da (for SEC-300) 20,000 – 500,000 Da (for SEC-500) |
| Theoretical plate number per meter |
100,000 | Used with | Guard columns are optional but not necessary |
Applications:
Determination of molecular weight and molecular weight distribution of unfractionated heparin.
Compendial methods:
- USP <2027> Heparin Sodium, Section D. Molecular Weight Determinations
- USP <1511> LMWH Sodium, Section D. Molecular Weight Distribution and Weight-Average Molecular Weight
- USP <209> Low Molecular Weight Heparin Molecular Weight Determinations
- EP <0828> Heparins, Low-molecular-mass, Section C. Size-exclusion chromatography
- CP 2015 Heparin sodium, Molecular weight and its distribution
Order information:
| Cat. # | Description | Qty. |
|---|---|---|
| B213-050030-07830S-HP | BioCore SEC-300HP Analytical Column, 300 Å, 5 μm, 7.8×300 mm | Each |
| B213-050050-07830S-HP | BioCore SEC-500HP Analytical Column, 500 Å, 5 μm, 7.8×300 mm | Each |
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