BioCore SEC-300HP & SEC-500HP Columns for Unfractionated Heparins


The combination of BioCore SEC-300HP and SEC-500HP analytical columns is recommended for molecular weight determinations of unfractionated heparin.

Description

Molecular weight (MW) and its distribution are important characteristics of heparins, a family of anticoagulants with polydisperse, heterogeneous polysaccharide chains. Current compendial methods for heparin molecular weight determinations involve size-exclusion chromatography (SEC).

Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution.

The combination of BioCore SEC-300HP and SEC-500HP analytical columns is recommended for molecular weight determinations of unfractionated heparin.

System suitability for unfractionated heparin

Heparin polysaccharide chains are hard to separation. With theoretical plate number per meter > 100,000, BioCore SEC-HP series SEC columns feature great column performance that can address this separation issue.

Chromatogram of unfractionated heparin
Chromatogram of unfractionated heparin

With BioCore SEC-300HP and SEC-500HP analytical columns, a correlation coefficient great than 0.9999 can be easily obtained in the calibration curve relating molecular weight to retention time.

Calibration curve with great correlation
Calibration curve with great correlation

Great durability with over 100 injections

Heparin samples are very complex and challenge column durability. As shown in the figure bellow, with BioCore SEC-150HP analytical column, minimal variation between 30 injections of LMWHs samples can be observed.

Overlay of chromatograms of 30 injections, showing the first 16 injections
Overlay of chromatograms of 30 injections, showing the first 16 injections

According to feedbacks from customers, BioCore SEC-150HP can be used over 100 injections. This durability reduces your cost per injection to 1/3 to 1/4, compared to SEC columns from other vendors.

Properties:

Separation mechanism Size-exclusion chromatography
(SEC)
Packaging type USP L59
I.D. 7.8 mm Length 300 mm
Matrix Monodisperse silica particle Functional group Diol
Pore size 300 Å (for SEC-300HP)
500 Å (for SEC-500HP)
Particle size 5 μm
pH range 2 – 8 Maximum pressure 6,000 psi
Calibration range
(globular proteins)
10,000 – 1,000,000 Da (for SEC-300)
20,000 – 2,000,000 Da (for SEC-500)
Calibration range
(glycosaminoglycans)
5,000 – 150,000 Da (for SEC-300)
20,000 – 500,000 Da (for SEC-500)
Theoretical plate number
per meter
100,000 Used with Guard columns are optional but not necessary

Applications:

Determination of molecular weight and molecular weight distribution of unfractionated heparin.

Compendial methods:

  • USP <2027> Heparin Sodium, Section D. Molecular Weight Determinations
  • USP <1511> LMWH Sodium, Section D. Molecular Weight Distribution and Weight-Average Molecular Weight
  • USP <209> Low Molecular Weight Heparin Molecular Weight Determinations
  • EP <0828> Heparins, Low-molecular-mass, Section C. Size-exclusion chromatography
  • CP 2015 Heparin sodium, Molecular weight and its distribution

Order information:

Cat. # Description Qty.
B213-050030-07830S-HP BioCore SEC-300HP Analytical Column, 300 Å, 5 μm, 7.8×300 mm Each
B213-050050-07830S-HP BioCore SEC-500HP Analytical Column, 500 Å, 5 μm, 7.8×300 mm Each

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