Heparinase I, for EP Method


Asnail provides heparinase I for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method.

SKU: AS00-2519-EP Category: Tags: , , , ,

Description

Heparinase I (heparin lyase I) cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1).

Asnail provides heparinase I for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method.

System suitability required by the European Pharmacopoeia Method:

– peak area ratio: maximum 1.15 for the peaks due to 1,6-anhydro ΔIS-IS and 1,6-anhydro ΔIS in the chromatogram obtained with reference solution (b);

– maximum 0.02 for the peaks due to ΔIS and reduced ΔIS in the chromatogram obtained with reference solution (c);

– resolution: minimum 1.5 between the peaks due to reduced ΔIA and 1,6-anhydro ΔIS in the chromatogram obtained with refer-ence solution (c);

– the content of 1,6-anhydro derivatives in heparin sodium CRS is within 1.5 per cent of the assigned content.

Properties:

Origin Flavobacterium Heparinum Purity (HPLC) ≥ 99%
CAS Number 9025-39-2 Specific Activity ≥ 400 IU/mg (with heparin)
EC Number 4.2.2.7 Concentration 10 IU/mL
Molecular Weight 42.8 kDa Storage Temperature  -20 °C

Applications:

Asnail™ heparinase I is used for determining 1,6-anhydro derivatives in heparin sodium.

Order information:

Cat. # Description Qty.
AS00-2519-EP Heparinase I, from Flavobacterium Heparinum,
for the European Pharmacopoeia Method
0.1 IU

Related Products:

Cat. # Description
AS00-2519 Heparinase I, from Flavobacterium Heparinum
AS00-6512 Heparinase II, from Flavobacterium Heparinum
AS00-8891 Heparinase III, from Flavobacterium Heparinum
AS00-6512-EP Heparinase II-EP, from Flavobacterium Heparinum
AS00-8891-EP Heparinase III-EP, from Flavobacterium Heparinum

Asnail Chinese Website:www.asnailtech.com.cn

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