Asnail Products for 1,6-Anhydro Derivative Test

The percentage of 1,6-anhydro species is a unique characteristics of enoxaparin sodium, one of low-molecular-weight heparins (LWMHs) that have been widely used as anticoagulants.

1,6-Anhydro Structure in Enoxaparin Sodium

How to determine this structure?

Procedures to determine levels of 1,6-anhydro structure are listed in pharmacopeias like EP <1097> and USP <207>.

To determine the percentage of 1,6-anhydro species, enoxaparin sodium samples are first degraded via a combination of heparinases I, II and III, followed by reduction via sodium borohydride. The degraded and reduced sample solutions are injected into a HPLC system for analysis.

Typical Chromatogram of Enoxaparin Sodium

Successful analysis of this unique characteristics in enoxaparin sodium heavily depends on the choice of right HPLC columns and heparinases.

Why choose Asnail?

For analysis of 1,6-anhydro species in enoxaparin sodium, Asnail offers heparinases, heparin-derived unsaturated disaccharides, and SAX columns.

Heparinases are the most important and expensive reagents used in QA of enoxaparin sodium. Asnail’s native heparinases feature great stability and purity. By now, Asnail’s heparinases have served many customers whose procedures are in compliance with EN and USP.

Heparinase I, II, and III for Degrading Enoxaparin Sodium

To identify peaks in chromatograms, Asnail provides heparin unsaturated disaccharides as reference materials which are prepared by degrading heparin and/or heparan sulfate via heparinases.

Heparin Disaccharides for Peak Identification

For HPLC columns, Asnail has tested and selected the Ultisil XB-SAX analytical column, which has proved to provide better performance than previously available columns. This column is co-developed with Welchmat, a long-time parter of Asnail.

XB-SAX Column for Analysis of 1,6-Anhydro Structure

Product links

Heparinases from Flavobacterium heparinum

Heparin-derived unsaturated disaccharides

Ultisil XB-SAX column for 1,6-anhydro test

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