Description
Molecular weight (MW) and its distribution are important characteristics of heparins, a family of anticoagulants with polydisperse, heterogeneous polysaccharide chains. Current compendial methods for heparin molecular weight determinations involve size-exclusion chromatography (SEC).
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution.
The combination of BioCore SEC-150HP analytical column and SEC-150 guard column is recommended for molecular weight determinations of low-molecular-weight heparins, such as enoxaparin sodium.
Better peak resolution for low-molecular-weight heparins
Heparin polysaccharide chains are hard to separation. With BioCore SEC-150HP analytical column, better resolution between adjacent peaks are obtained.
Baseline separation is almost achieved between the best-separated peaks (MW 1,200 and 600, respectively)
Great durability with over 100 injections
Heparin samples are very complex and challenge column durability. As shown in the figure bellow, with BioCore SEC-150HP analytical column, minimal variation between 30 injections of LMWHs samples can be observed.
According to feedback from customers, BioCore SEC-150HP analytical column can be used over 100 injections. This durability reduces your cost per injection to 1/3 to 1/4, compared to SEC columns from other vendors.
Properties:
| Separation mechanism | Size-exclusion chromatography (SEC) |
Packaging type | USP L59 |
|---|---|---|---|
| I.D. | 7.8 mm | Length | 300 mm |
| Matrix | Monodisperse silica particle | Functional group | Diol |
| Pore size | 150 Å | Particle size | 5 μm |
| pH range | 2 – 8 | Maximum pressure | 6,000 psi |
| Calibration range (globular proteins) |
5,000 – 150,000 Da | Calibration range (glycosaminoglycans) |
1,000 – 50,000 Da |
| Theoretical plate number per meter |
100,000 | Used with | BioCore SEC-150 Guard Column |
Applications:
Determination of molecular weight and molecular weight distribution of low-molecular-weight heparins.
Compendial methods:
- USP <2027> Heparin Sodium, Section D. Molecular Weight Determinations
- USP <1511> Enoxaparin Sodium, Section D. Molecular Weight Distribution and Weight-Average Molecular Weight
- USP <209> Low Molecular Weight Heparin Molecular Weight Determinations
- EP <0828> Heparins, Low-molecular-mass, Section C. Size-exclusion chromatography
Order information:
| Cat. # | Description | Qty. |
|---|---|---|
| B213-050015-07830S-HP | BioCore SEC-150HP Analytical Column, 150 Å, 5 μm, 7.8×300 mm | Each |
| B213-050015-04601S-HP | BioCore SEC-150 Guard Column, 5 μm, 4.6×10 mm | Each |
| Guard-04601S | BioCore Guard Column Cartridge | Each |
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