Description
Heparinase I (heparin lyase I) cleaves heparin and to a lesser extent heparan sulfate (ratio approx. 3:1).
Asnail provides heparinase I for determining 1,6-anhydro derivatives in heparin sodium. This enzyme has proven to meet the system suitability required by the European Pharmacopoeia method.
System suitability required by the European Pharmacopoeia Method:
– peak area ratio: maximum 1.15 for the peaks due to 1,6-anhydro ΔIS-IS and 1,6-anhydro ΔIS in the chromatogram obtained with reference solution (b);
– maximum 0.02 for the peaks due to ΔIS and reduced ΔIS in the chromatogram obtained with reference solution (c);
– resolution: minimum 1.5 between the peaks due to reduced ΔIA and 1,6-anhydro ΔIS in the chromatogram obtained with refer-ence solution (c);
– the content of 1,6-anhydro derivatives in heparin sodium CRS is within 1.5 per cent of the assigned content.
Properties:
| Origin | Flavobacterium Heparinum | Purity (HPLC) | ≥ 99% |
|---|---|---|---|
| CAS Number | 9025-39-2 | Specific Activity | ≥ 400 IU/mg (with heparin) |
| EC Number | 4.2.2.7 | Concentration | 10 IU/mL |
| Molecular Weight | 42.8 kDa | Storage Temperature |  -20 °C |
Applications:
Asnailâ„¢ heparinase I is used for determining 1,6-anhydro derivatives in heparin sodium.
Order information:
| Cat. # | Description | Qty. |
|---|---|---|
| AS00-2519-EP | Heparinase I, from Flavobacterium Heparinum, for the European Pharmacopoeia Method |
0.1 IU |
Related Products:
Asnail Chinese Website:www.asnailtech.com.cn
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